DNA microarray based rapid drug susceptibility test for Mycobacterium leprae
نویسندگان
چکیده
منابع مشابه
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
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Understanding the molecular basis underlying the hallmark features of Mycobacterium leprae, such as its parasitism and extraordinarily slow growth rate, has stimulated research into the biology of this pathogen for decades, but it is through the completion of its genome sequence that a quantum leap of progress has been achieved. 1 ln this review, we analyse the genes in M. leprae that are invol...
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Managing drug-resistant Mycobacterium tuberculosis requires drug susceptibility testing, yet conventional drug susceptibility testing is slow, and molecular testing does not yield results for all antituberculous drugs. We addressed these challenges by utilizing real-time PCR of mycobacteriophage D29 DNA to evaluate the drug resistance of clinical M. tuberculosis isolates. Mycobacteriophages inf...
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ژورنال
عنوان ژورنال: Japanese journal of leprosy
سال: 2006
ISSN: 1342-3681,1884-314X
DOI: 10.5025/hansen.75.271